![]() If the use of original supernate sample or low dilution (<5 fold) are necessary due to the expected low concentration of antigen supernates need be adjust to neutral pH condition before assay. Note: Grossly hemolyzed samples are not suitable for use in this assayĬell Culture Supernates – Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃ or lower temperature. For long-term storage please store at -20☌. The samples may be kept at 2 – 8☌ for up to three days. In cases where matrix interferences is under or over observed, the samples may be diluted with Assay Diluent accordingly. Samples should be diluted 1:100 (v/v) for optimal recovery, (for example 1 ul sample + 99 ul (1X) Assay Diluent) prior to assay. Repeated freezing and thawing should be avoided. Lipaemic, hemolytic or contaminated samples should not be run. Serum is separated after clotting by centrifugation. Samples should be run at a number of dilutions to ensure accurate quantitation. Specimens should be clear and non-hemolyzed. ![]() Color development is then stopped by addition of stop solution. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Anti-Human Anti-SARS-CoV-2 S1 (2019-nCoV) in the sample. After incubation the wells are washed and followed by addition of HRP-conjugated Detection IgG Antibody into each well and incubated to form a complex. Samples and standards are pipetted into microwells and Antibodies to Human Anti-SARS-CoV-2 (2019-nCoV) present in the sample are bound by the protein antigen. SARS-CoV-2 Spike S1 recombinant protein is precoated onto microwells. ![]() The method employs indirect sandwich ELISA technique. The Anti-SARS-CoV-2 (COVID-19) IgG Antibody to Spike Protein S1 Quantitative Titration ELISA Assay Kit is For Research Use OnlyĪlternative Name: Coronavirus, SARS-CoV, Covid-19 Anti-SARS-CoV-2 (COVID-19) IgG Antibody to Spike Protein S1 Quantitative Titration ELISA Assay Kit ![]()
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